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Recent advances in mouse genetics and the development of very precise assays for cell phenotypes have enabled quantification of the kinetics of growth and turnover of subsets of natural killer (NK) cell populations. Yet, the most important function of NK cells, their ability to lyse tumor cells and infected cells, remains the most difficult to measure. This is due to the fact that a single NK cell can kill tumor cells or virus-infected cells that are targets or resistant to the killing process, respectively. Here, we used the methods of generalized linear models to quantify the contribution of the primary NK cell pool (both mature and immature) and the effector NK cell pool (mature, effector, and immature) to tumor cell destruction by a standard murine cytomegalovirus infection of mice with an intact immune system. We describe a series of models using two YAC T cell receptor gene mutants (LY75.1(+) and LY75.1(-)) as targets to investigate the interaction of host and viral factors controlling the formation of cytotoxic effector molecules from NK cells
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tag less java hibernate 13crack password 12Preoperative Evaluation of Inferior Vena Cava Thrombosis: A Comparison of Conventional CT and New Dual-Energy CT Scanning Techniques. To evaluate the value of a new dual-energy CT (DE-CT) technique for the preoperative evaluation of inferior vena cava (IVC) thrombosis and to compare it with conventional CT (cCT). In this prospective cohort study, a total of 33 consecutive patients (21 men, 12 women; mean age ± SD, 59.2 ± 12.6 years; age range, 26-79 years) with suspected deep vein thrombosis (DVT) and contraindication or refusal of anticoagulant therapy were enrolled. DE-CT scans of the venous system were obtained at 40 and 70 kVp within 60 seconds after a bolus of 150 mL contrast material (total dose, 4.2 mL/kg). Two radiologists subjectively evaluated the images and assigned a perfusion score to the suprahepatic and infrahepatic IVC from 0 (most reduced) to 5 (normal). Blood counts were determined, and clots in the IVC or femoral veins were verified by ultrasound or intravenous contrast injection. For 30 patients (90.9%), conventional CT (cCT) using 120 kVp was obtained. In 22 patients (66.7%), preoperative duplex ultrasound examination confirmed the presence of a cIVC thrombus. After combining the results of cCT and ultrasound, 14 patients (42.4%) had either normal perfusion scores (n = 5) or a score of 0 (n = 9). The DE-CT technique yielded normal perfusion scores in all 22 patients and a score of 3 or 4 in 13 of the 14 patients (92.9%) in whom DVT was excluded with ultrasound (P Lorenzo Savona Lorenzo Savona (born 30 April 1972 in Trissino, province of Como